Since the inception of CRISPR technology in 2012, TP53 has emerged as the most extensively studied gene, with 66 knock-out instances across 3782 CRISPR-related articles. Among 2479 individually targeted genes in mammalian cells, TP53 stands out, knocked out in 41 host cell lines. Notably, the hTERT-RPE1 cell line has been used six times for TP53 knock-outs, highlighting its significance in studying cell division and DNA repair. This data unveils TP53’s prevalence in CRISPR research and its pivotal role in cellular investigations.
